miR-31 Functions as an Oncomir Which Promotes Epithelial-Mesenchymal Transition via Regulating BAP1 in Cervical Cancer

Figure 2

BAP1 is a direct downstream target of miR-31. (a) Putative miR-31 binding site of human BAP13′-UTR containing wild type (WT) and mutant (MUT). Either WT or MUT BAP1 3′-UTR was subcloned into the dual-luciferase reporter plasmid. (b) Relative luciferase activity of the BAP1 wild type and mutant BAP1 3′-UTR. ((c) and (d)) RT-PCR and Western blot assay showed the alteration of BAP1 and miR-31 in cervical cancer cells. Experiments were performed in triplicate. (e) RT-PCR results showed BAP1 expression in 31 cervical cancer tissues and adjacent normal tissues. (f) Inverse correlation between miR-31 and BAP1. ,.

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