Research Article
In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering
Figure 4
Effect of ALN concentration in fibrin/ALN gels on cell viability. Human mesenchymal stem cells (hMSCs) were embedded in fibrin/ALN gels containing various concentrations of ALN. After 3 days of cultivation, live/dead staining was performed. Most of the hMSCs were viable and retained a fibroblast-like morphology (stained by calcein AM, shown in green) in fibrin gels and 10−7, 10−6, and 10−5 M ALN composite fibrin gels. In contrast, when cells were embedded in 10−4 M ALN fibrin/ALN gel, a large number of cells were found to be dead or unhealthy (stained by EthD-1, shown in red) and cells were poorly distributed and had a rounded cellular morphology.