Research Article
In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering
Figure 5
Effect of ALN concentration in fibrin/ALN gels (a) on alkaline phosphatase (ALP) activity (ND, not detected) and (b) calcium accumulation. Human mesenchymal stem cells (hMSCs) were embedded in the fibrin/ALN gels containing various concentrations of ALN. ALP activity assay and alizarin red S staining were performed after 7 and 14 days of cultivation, respectively. (c) In addition, after 7 days of cultivation, real-time PCR was performed for several osteoblast marker genes and cells cultured in fibrin/ALN gels containing 10−7, 10−6, and 10−5 M ALN, and osteoblast differentiation was significantly increased. The data shown are the means ± standard deviation (SD) of three independent experiments. and compared with control.
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