Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa

Table 1

Purification of α-amylases extracted from leaves and stems of Pergularia tomentosa.

Step	Specific activity (U/mg)	Purification (fold)	Yield (%)
Leaves
Crude extract	0.547	-	-
Heat treatment	0.558	1.012	76.905
Anion exchange chromatography
25–149 mM NaCl	6.463	11.821	17.19
225–398 mM NaCl	2.672	4.887	38.333
402–450 mM NaCl	3.787	6.927	23.81
Stems
Crude extract	0.554	-	-
Heat treatment	0.619	1.117	84.567
Anion exchange chromatography
290–435 mM NaCl	5.41	9.756	28.224
620–690 mM NaCl	6.048	10.924	27.272