First Characterization of the Neospora caninum Dense Granule Protein GRA9

Figure 6

(a) shows that NcGRA9 belongs to the “excreted secreted antigens” (ESA) of N. caninum. The amount of NcGRA9 and the NcGRA7 control protein accumulated over time. In contrast NcSAG1 control protein was not present in ESA. In (b) sera from N. caninum infected cattle (diluted 1 : 50) were tested for their recognition of recombinant NcGRA9 protein (5 μg) via Western Blot analyses. Three out of four cattle sera contained antibodies against recombinant NcGRA9 (black arrows). NLA: Neospora lysate antigen.

(a)

(b)