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BioMed Research International
Volume 2017 (2017), Article ID 6757898, 10 pages
https://doi.org/10.1155/2017/6757898
Clinical Study

Metastasis-Associated Protein 1 Is Involved in Angiogenesis after Transarterial Chemoembolization Treatment

1College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China
2Laboratory of Molecular Medicine, First People’s Hospital Affiliated to Huzhou University, Huzhou 313000, China
3Department of Hepatobiliary Surgery, First People’s Hospital Affiliated to Huzhou University, Huzhou 313000, China
4Department of Nephrology, First People’s Hospital Affiliated to Huzhou University, Huzhou 313000, China
5Moores Cancer Center, University of California, San Diego, CA 92037, USA
6Department of Ultrasound, First People’s Hospital Affiliated to Huzhou University, Huzhou 313000, China
7Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China

Correspondence should be addressed to Jianming Guan; moc.621@0039naug and Suhong Chen; nc.ude.tujz@gnohusnehc

Received 22 December 2016; Accepted 23 March 2017; Published 15 May 2017

Academic Editor: Melchiorre Cervello

Copyright © 2017 Tao Xue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Transarterial chemoembolization (TACE), a well-established treatment for unresectable hepatocellular carcinoma (HCC), blocks the arterial blood supply to the tumor, which can be short-lived as development of collateral neovessels, leading to the failure of treatment. Metastasis-associated protein 1 (MTA1) is involved in development of tumors and metastases. However, the role of MTA1 in angiogenesis is still obscure. Methods. We detected the expression of MTA1 and hypoxia-inducible factor-1α (HIF-1α) and microvessel density (MVD) value in liver tumor tissues and tumor periphery before and after TACE treatment. Hepatocellular carcinoma cell line HepG2, tube formation assay, and chorioallantoic membrane (CAM) assay were applied to explore the mechanism of MTA1 in angiogenesis. Results. We found that expression of MTA1 increased after TACE treatment, especially in tumor periphery, which was accompanied by markedly elevated MVD value, indicating a significant correlation between MTA1 and MVD value. Moreover, MTA1 contributed to neovascularization of residual tumors. Cellular experiments further revealed that MTA1 increased the stability and the expression of HIF-1α, and overexpression of MTA1 enhanced tube formation and neovessels of chick embryos. Conclusions. MTA1 is an active angiogenic regulator; our results shed light on better understanding in neovascularization, which are helpful to predict prognosis of TACE, and provide evidences for intervention to improve therapeutic effects on HCC.