Quantitative Serum Proteomic Analysis of Essential Hypertension Using iTRAQ Technique

Figure 2

Reproducibility of proteomic analysis. The abscissa represents different variation levels; the left ordinate represents the number of quantitative proteins at different variation levels, and the right ordinate represents the accumulation ratio of total quantitative proteins at different variation levels. (a) and (b) display a comparison of the intragroup; for example, (a) shows that a difference less than variation of 0.1 could be observed in approximately 61.0% of the proteins (A1/A2) and a difference less than variation of 0.5 could be observed in more than 95.0% of the proteins. Besides, (c) exhibits the reproducibility of the comparison group. The mean CV of this comparison group was 8.3%, and when CV was 30%, the coverage ratio was up to 96% of all quantitative proteins between two comparison groups (A1 versus B1/A2 versus B2).

(a)

(b)

(c)