Research Article
Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRH/LH Secretion during the Follicular Phase of the Estrous Cycle
Table 3
Effect of lipopolysaccharide (LPS; 400 ng/kg; iv.) and acetylcholinesterase inhibitors: Donepezil (2.5 mg/animal; iv.) and Neostigmine (0.5 mg/animal; iv.) injections on the relative gene expression (mean ± SEM; = 6 animals per group) of gonadotropin-releasing hormone (GnRH) in the hypothalamus of ewes during the follicular phase of the estrous cycle. POA: the preoptic area; AHA: the anterior hypothalamus; MBH: the medial basal hypothalamus; ME: the median eminence; control: group injected with saline; Don.: group treated with Donepezil; Neo.: group injected with Neostigmine; LPS: group which received the endotoxin injection; Don. + LPS: group treated with both Donepezil and LPS; Neo. + LPS: group treated with both Neostigmine and LPS. In all hypothalamic structures gene expression data were normalised to the average relative level of gene expression in the control ewes, which was set to 1.0. Different capital letters indicate significant () differences according to a two-way ANOVA followed by Fisher’s post hoc test.
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