Fabrication and Evaluation of Electrospun, 3D-Bioplotted, and Combination of Electrospun/3D-Bioplotted Scaffolds for Tissue Engineering Applications

Figure 4

Cell spreading and proliferation of human adipose-derived stem cells throughout scaffolds. Cells were cultured for 21 days in 3D-bioplotted, electrospun, or combination of 3D-bioplotted/electrospun scaffolds, fixed, and stained (actin = red; nuclei = blue). Superficial and cross-sectional views show cells present both on the surfaces of the scaffolds (superficial) and throughout the centers of the scaffolds on the 3D-bioplotted and combined scaffolds (cross-section). Human ASC exhibited steady proliferation over 21 days of culture on all scaffold types as indicated by AlamarBlue (% AB reduction). Bars indicate mean ± SEM (; ).

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