Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated

Table 1

Differentially expressed proteins in the ovary on days 55 and 90 of gestation.

Number	Protein IDs	Mascot score	Mr	PI	Fold change	Coverage	Protein name
Anatomical structure development
S30	F1RS36	478	70323	5.21	−2.94988	33%	78 kDa glucose-regulated protein
S1	F1SDX6	488	78202	5.19	+1.60756	28%	High similarity protein-glutamine gamma-glutamyltransferase 2
S15	F1SGP8	169	38756	4.74	+4.17177	24%	High similarity reticulocalbin-1
S18	I3LMU6	231	37602	4.88	+5.07398	32%	High similarity reticulocalbin-3
S16	I3LMU6	194	37602	4.88	+10.8714	19%	High similarity reticulocalbin-3
S32	F1RKM0	346	66674	5.08	−2.016	38%	High similarity lamin-B1
Cytoskeleton organization
S22	TBB5	194	50095	4.78	+3.63517	16%	Tubulin beta chain
S28	GELS	118	85065	5.93	−2.19131	8%	Gelsolin
S39	TPM4	314	28619	4.67	−1.65209	39%	Tropomyosin alpha-4 chain
Response to stress
S19	F1SRK6	282	92633	5.03	+10.0021	13%	Endoplasmin
S29	F1RQU2	120	83543	4.96	−2.51883	18%	High similarity heat shock protein HSP 90-beta
S6	F1SMZ7	162	61266	5.61	+1.83048	20%	High similarity 60 kDa heat shock protein, mitochondrial
S10	I3LQS0	89	49512	5.38	+5.17595	14%	High similarity heterogeneous nuclear ribonucleoprotein K
S5	I3LNG8	327	48248	7.01	+1.61547	44%	High similarity stress-induced phosphoprotein 1
S31	F1SA70	329	70065	5.51	−4.51308	25%	High similarity heat shock-related 70 kDa protein 2
Cell differentiation
S36	C7AI81	367	42381	5.23	−1.63319	36%	Actin alpha 2
S27	F1S596	188	61481	4.36	−1.64581	11%	High similarity glucosidase 2 subunit beta
S20	I3LVD5	377	42108	5.31	+5.52608	34%	Actin, cytoplasmic 1
S21	I3LVD5	294	42108	5.31	+1.7995	33%	Actin, cytoplasmic 1
S23	VIME	408	53692	5.06	+2.45187	36%	Vimentin
Biosynthetic process
S35	B26/I3LK59	240	38173	8.93	−2.02688	16%	Enolase
S37	F1SQ06	150	21243	4.83	−3.32526	31%	High similarity spermine synthase
Cell proliferation
S25	I3LQ79	477	99973	5.53	−3.23725	23%	High similarity major vault protein
S26	I3LQ79	566	99973	5.53	−25.8052	32%	High similarity major vault protein
S40	APOA1	177	30307	5.48	−1.62505	40%	Apolipoprotein A-I
Transport
S3	FETA	390	70405	5.47	+2.00804	30%	Alpha-fetoprotein
S4	A2THZ2	325	71550	5.92	+32.0392	29%	Albumin
S42	A2THZ2	488	71550	5.92	−2.71787	35%	Albumin
Protein folding and maturation
S2	F1S0M9	174	34453	8.24	+6.61563	33%	Peptidyl-prolyl cis-trans isomerase
S34	F1RP17	246	61756	6.79	−2.44313	19%	T-complex protein 1 subunit gamma
Small molecule metabolic process
S13	Q2HPK3	381	33321	5.86	+1.86412	35%	Tubulin alpha-3 chain
S41	A6M930	193	46601	5.33	+1.75218	32%	Eukaryotic translation initiation factor 4A isoform 2
Others
S7	A1AT	125	47449	5.54	+2.38373	9%	Alpha-1-antitrypsin
S8	F1RIP6	188	53170	5.02	+2.21427	30%	High similarity nucleobindin-1
S9	F1SCD0	103	46782	5.8	+2.93238	17%	Uncharacterized protein
S11	F1S1U5	164	47140	4.7	+11.3392	17%	High similarity Golgi reassembly-stacking protein 2
S12	F1SME6	331	56996	6	+3.44637	28%	Uncharacterized protein
S14	F1SFI6	310	43561	7.87	+3.28303	32%	Uncharacterized protein
S17	KCRB	129	11670	5.19	+2.12203	28%	Creatine kinase B-type
S24	Q1T7A9	82	10639	5	−1.76143	27%	Type VI collagen alpha-1 chain
S33	I3L954	184	60941	5.55	−1.78172	14%	Uncharacterized protein
S38	F2Z5C1	229	33193	4.95	−1.94943	36%	Annexin
S43	I3L954	64	60941	5.55	−2.06063	9%	Uncharacterized protein

Mr: relative molecular mass; PI: isoelectric point. Symbols (+) and (−) denote an increase and a decrease, respectively, at day 55 of gestation of fetal ovaries, when compared with day 90 of gestation of fetal ovaries. High similarity indicated that those proteins are from mouse databases (https://www.ncbi.nlm.nih.gov/protein/?term=txid10088[Organism:exp]).