Long Noncoding RNA PVT1 as a Novel Diagnostic Biomarker and Therapeutic Target for Melanoma

Figure 4

Overexpression of PVT1 enhances melanoma cells proliferation, cell cycle progression, and migration. (a) PVT1 expression levels in PVT1 stably overexpressed and control A375 cells were measured by qPCR. (b) The effect of PVT1 overexpression on A375 cells proliferation was measured by CCK-8 assays. (c) The effect of PVT1 overexpression on A375 cells proliferation was measured by EdU incorporation assays. Scale bars, 100 μm. (d) The effect of PVT1 overexpression on A375 cell cycle distribution was measured by flow cytometry. (e) The effect of PVT1 overexpression on A375 cells migration was measured by transwell assays. Scale bars, 100 μm. (f) Western blot analysis of MYC protein in PVT1 stably overexpressed and control A375 cells. Data are presented as mean ± SD. , , by Student’s -test.

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