Long Noncoding RNA PVT1 as a Novel Diagnostic Biomarker and Therapeutic Target for Melanoma

Figure 5

Depletion of PVT1 inhibits melanoma cells proliferation, cell cycle progression, and migration. (a) PVT1 expression levels in PVT1 stably depleted and control A375 cells were measured by qPCR. (b) The effects of PVT1 depletion on A375 cells proliferation were measured by CCK-8 assays. (c) The effects of PVT1 depletion on A375 cells proliferation were measured by EdU incorporation assays. Scale bars, 100 μm. (d) The effects of PVT1 depletion on A375 cell cycle distribution were measured by flow cytometry. (e) The effects of PVT1 depletion on A375 cells migration were measured by transwell assays. Scale bars, 100 μm. (f) Western blot analysis of MYC protein in PVT1 stably depleted and control A375 cells. Data are presented as mean ± SD. , , , and by Student’s -test.

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