BioMed Research International / 2017 / Article / Tab 1

Research Article

Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 1

Composition of various media used to cultivate procured strains for nitrilase production.

Name of the organismMTCC numberComposition ()pHGrowth temperature

Shimwellia blattae
ATCC 29907
4155Beef extract: 1.0 g
Yeast extract: 2.0 g
Peptone: 5.0 g
NaCl: 5.0 g
Agar: 15.0 g
7.0–7.537°C

Runella slithyformis
ATCC 29530
9504Glucose: 1.0 g
Peptone: 1.0 g
Yeast extract: 1.0 g
Agar: 15.0 g
Glucose: 4.0 g
7.0–7.526°C

Geodermatophilus obscurus
DSM 43160
4040Yeast extract: 4.0 g
Malt extract: 10.0 g
CaCO3: 2.0 g
Agar: 12.0 g
7.2–7.528°C

Nocardiopsis dassonvillei
DSM 43111
1411Yeast extract: 4.0 g
Malt extract: 1.0
Glucose: 4.0 g
Agar: 20.0 g
7.2–7.428°C

Streptomyces albus
J1074
1138Yeast extract: 4.0 g
Malt extract: 1.0 g
Glucose: 4.0 g
Agar: 20.0 g
7.2–7.425°C

Flavobacterium indicum
DSM 17447
6936Tryptic soy broth with agar
(TSBA-100)
7.3–7.530°C

Saccharomonospora viridis
ATCC 15386
320Yeast extract: 4.0 g
Malt extract: 1.0 g
Glucose: 4.0 g
Agar: 20.0 g
7.2–7.445°C

Sphingopyxis alaskensis
DSM 13593
7504Beef extract: 1.0 g
Yeast extract: 2.0 g
Peptone: 5.0 g
NaCl: 5.0 g
Agar: 15.0 g
7.0–7.530°C

Gluconacetobacter diazotrophicus
ATCC 49037
1224Yeast extract: 5.0 g
Peptone: 3.0 g
Mannitol: 25.0 g
Agar: 15.0 g
7.0–7.328°C

Escherichia coli
BL21 (DE3)
Yeast extract: 5.0 g
NaCl: 10.0 g 
Casein enzymatic hydrolysate: 10.0 g
7.0–7.537°C

Negative control.