BioMed Research International / 2017 / Article / Tab 6

Research Article

Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 6

Nitrilase activity of in silico predicted microbial sources of nitrilases assayed using common aliphatic, aromatic, aryl aliphatic, and KCN as substrate.

Organisms Substrates
ValeronitrileBenzonitrileMandelonitrileIsobutyronitrileAdiponitrile2-CyanopyridinePropionitrileAcrylonitrileKCN

Streptomyces albus
J1074
0.00150.0027ND0.0014NDNDNDNDND

Nocardiopsis dassonvillei
DSM 43111
ND0.00400.0024NDNDNDNDNDND

Geodermatophilus obscurus
DSM 43160
ND0.00430.0021NDNDNDNDNDND

Shimwellia blattae
ATCC 29907
0.0028ND0.00160.0019NDNDNDNDND

Runella slithyformis
ATCC 29530
ND0.02970.01520.0095NDND0.0169NDND

Gluconacetobacter diazotrophicus
ATCC 49037
ND0.00160.00200.00510.0048NDNDNDND

Sphingopyxis alaskensis
DSM 13593
ND0.00073ND0.00240.00075NDNDNDND

Saccharomonospora viridis
ATCC 15386
NDNDND0.0030NDNDNDNDND

Flavobacterium indicum
DSM 17447
NDNDNDNDNDNDNDND0.25

Escherichia coli
BL21 (DE3)
NDNDNDNDNDNDNDNDND

Expressed as µmole of ammonia released/min/mg dcw under the assay conditions; ND = not detected; negative control.

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