Mining of Microbial Genomes for the Novel Sources of Nitrilases
Table 6
Nitrilase activity of in silico predicted microbial sources of nitrilases assayed using common aliphatic, aromatic, aryl aliphatic, and KCN as substrate.
Organisms
Substrates
Valeronitrile
Benzonitrile
Mandelonitrile
Isobutyronitrile
Adiponitrile
2-Cyanopyridine
Propionitrile
Acrylonitrile
KCN
Streptomyces albus J1074
0.0015
0.0027
ND
0.0014
ND
ND
ND
ND
ND
Nocardiopsis dassonvillei DSM 43111
ND
0.0040
0.0024
ND
ND
ND
ND
ND
ND
Geodermatophilus obscurus DSM 43160
ND
0.0043
0.0021
ND
ND
ND
ND
ND
ND
Shimwellia blattae ATCC 29907
0.0028
ND
0.0016
0.0019
ND
ND
ND
ND
ND
Runella slithyformis ATCC 29530
ND
0.0297
0.0152
0.0095
ND
ND
0.0169
ND
ND
Gluconacetobacter diazotrophicus ATCC 49037
ND
0.0016
0.0020
0.0051
0.0048
ND
ND
ND
ND
Sphingopyxis alaskensis DSM 13593
ND
0.00073
ND
0.0024
0.00075
ND
ND
ND
ND
Saccharomonospora viridis ATCC 15386
ND
ND
ND
0.0030
ND
ND
ND
ND
ND
Flavobacterium indicum DSM 17447
ND
ND
ND
ND
ND
ND
ND
ND
0.25
Escherichia coli BL21 (DE3)
ND
ND
ND
ND
ND
ND
ND
ND
ND
Expressed as µmole of ammonia released/min/mg dcw under the assay conditions; ND = not detected; negative control.
