Effects of Cobalt Chloride, a Hypoxia-Mimetic Agent, on Autophagy and Atrophy in Skeletal C2C12 Myotubes

Figure 4

CoCl₂ promoted C2C12 cells protein degradation. QRT-PCR and Western blotting were used to determine the mRNA and protein levels of parkin (a and b) and atrogin-1 (c and d) in C2C12 cells treated with CoCl₂. The bands were quantified using Image J and the expression levels of parkin and atrogin-1 were normalized relative to tubulin. compared to control group.