Research Article
Effects of Electrostatic Field on Osteoblast Cells for Bone Regeneration Applications
Table 1
Primer information for real-time PCR.
| Gene | Accession number | Sequence | Melting temperature (°C) |
| GAPDH | NM_002046 | Forward: 5′-GAACATCATCCCTGCCTCTACTG-3′ Reverse: 5′-TCCGACGCCTGCTTCACC-3′ | 58.2 58.8 |
| COL1A1 | NM_000088 | Forward: 5′-CAGGGCGACAGAGGCATAAAG-3′ Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′ | 58.3 58.3 |
| OCN | NM_000711 | Forward: 5′-GCAGAGTCCAGCAAAGGTG-3′ Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′ | 55.5 55.7 |
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