Integron-Mediated Antibiotic Resistance in Acinetobacter baumannii Isolated from Intensive Care Unit Patients, Babol, North of Iran

Table 1

Primer sequences, PCR product sizes, and PCR programs for replication of class I, II, and III integrons.

Genes	Primer sequence ()	PCR product (bp)	PCR program	Reference
IntI-I	F-TCTCGGGTAACATCAAGG; R-AGGAGATCCGAAGACCTC	243	5 min at 94°C; 35 cycles (1 min at 94°C, 1 min at 53°C, and 30 sec at 72°C); 5 min at 72	[9]
IntI-II	F-TTATTGCTGGGATTAGGC; R-ACGGCTACCCTCTGTTATC	233	5 min at 94°C; 30 cycles (1 min at 94°C, 1 min at 50°C, and 1 min at 72°C); 10 min at 72°	[10]
IntI-III	F-AGTGGGTGGCGAATGAGTG; R-TGTTCTTGTATCGGCAGGTG	600	5 min at 94°C; 30 cycles (1 min at 94°C, 1 min at 50°C, and 1 min at 72°C); 10 min at 72°C	[10]