Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy

Figure 1

Renal cortex homogenates ((a) 40 μg proteins) and apical membranes ((b) 30 μg proteins) and renal medullae homogenates ((c) 40 μg proteins) and apical membranes ((d) 30 μg proteins) from kidneys of sham and BUO rats were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (5%) and blotted onto nitrocellulose membranes. NKCC2 (150 kDa) was identified using commercial polyclonal antibodies as described in Section 2. Kaleidoscope-prestained standards of molecular mass (Mr) corresponding to myosin (206.4 kDa) and to carbonic anhydrase (38.9 kDa) are indicated in the left of the figure. Sham levels were set at 100%. Each column represents mean ± SE from experiments carried out in triplicate on four different cortex and medulla homogenates and apical membranes preparations for each experimental group. versus sham, versus BUO-1, versus BUO-2, and versus BUO-7.

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