Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy

Figure 4

Urinary excretion of NKCC2 in sham and BUO rats. Urine samples were separated by SDS-PAGE (8.5%) and blotted onto nitrocellulose membranes. NKCC2 was identified using commercial polyclonal antibody as described in Section 2. The densitometric quantification of urine NKCC2 immunoblotting is expressed as the percentage of a ratio of arbitrary units relative to urinary creatinine concentration for each sample. Sham levels were set at 100%. Results are expressed as mean values ± SE. versus sham, versus BUO-1, versus BUO-2, and versus BUO-7.