BioMed Research International / 2017 / Article / Fig 1

Research Article

PEDF Is Associated with the Termination of Chondrocyte Phenotype and Catabolism of Cartilage Tissue

Figure 1

Detection of PEDF mRNA and protein in adult human joint tissues. Toluidine blue staining (a) depicts articular cartilage (white arrow), osteophytic cartilage (black arrowhead), subchondral bone (black arrow), and periarticular mesenchyme (open arrowhead). Inserts show higher magnifications of osteophytic cartilage. PEDF is detectable by immunostaining in osteophytic cartilage and the osteoblastic lining of subchondral bone (b). Correspondingly, MMP13 immunostaining is positive in osteophytic cartilage and subchondral bone (c). Quantitative RT-PCR analysis of PEDF (d) and MMP13 (e) in articular cartilage (AC), periarticular mesenchyme/periosteum (PM), osteophytic cartilage (OC), and subchondral bone (B). Detection of protein levels of PEDF and α-tubulin (loading control) by immunoblot analysis (f). Bars = 200 µm. ; .

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