Analysis of PEDF target genes and pathways in chondrocytes using RNA-Seq. Principal component analysis of RNA-Seq expression data demonstrate distinct separation between PEDF-stimulated and nonstimulated chondrocytes (a). Hierarchical clustered heatmap shows differentially expressed genes for each sample. A total of 613 genes (in red) are upregulated and 520 genes (in blue) are downregulated following PEDF treatment (b). IPA-determined enriched signaling pathways (c), disorders (d), and cellular functions (e) associated with PEDF treatment.