BioMed Research International / 2017 / Article / Tab 1 / Research Article
Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae Table 1 The primers used for genes replacement and PCR in this study.
Primers name Sequence of PCR primers Application of primers P1UFMGG-00623 ctcgagATTCGGGTCCTTCGTTAT For genes replacement P1URMGG-00623 gtcgacCCTCCCTCTGTTGTCTTGT For genes replacement P1DFMGG-00623 actagtGACCGTGATCGACCTTCC For genes replacement P1DRMGG-00623 gagctcATGCCCTCTTTGACTTGG For genes replacement P2UFMGG_00871 ggtacc TCGAGGGTTATCAAGCAA For genes replacement P2URMGG_00871 gtcgacAAATAGAAGCCGCCAGAC For genes replacement P2DFMGG_00871 gaattcGATGACGAGTTGCGATGT For genes replacement P2DRMGG_00871 tctagaGGGACCTGCTCTGTATCA For genes replacement P3UFMGG_06704 gggcccCCGTCATCACCTAACCAA For genes replacement P3URMGG_06704 ctcgagGAACAGCGTCGTCTCCAT For genes replacement P3DFMGG_06704 actagtGACCGTGATCGACCTTCC For genes replacement P3DRMGG_06704 gagctcATGCCCTCTTTGACTTGG For genes replacement P4UFMGG_00745 ctcgagGCGGGTCAAAGAGTGTATT For genes replacement P4URMGG_00745 gagctcGTCGTTGGGTATTGGGTC For genes replacement P4DFMGG_00745 gaattcCACTTCTTTCCCTGGTCG For genes replacement P4DRMGG_00745 gagctcTCCTCTGGAGCTTTCCTC For genes replacement P5UFMGG_04068 gggcccGGGGCAAGGTTCTCAAAG For genes replacement P5URMGG_04068 gtcgacAAGCGAGGTGGCAGGTAG For genes replacement P5DFMGG_04068 aagcttAGGTCGTAGACATACTGAGGT For genes replacement P5DRMGG_04068 gaattcAAGGCTGTAGATGGCTGA For genes replacement CP1FMGG-00623 ACTTGATGGCTAACCACTACTT For PCR screening CP1RMGG-00623 CCAATATGTCCGAGACGAT For PCR screening CP2FMGG_00871 CCCATTGATACTGCGGTTAG For PCR screening CP2RMGG_00871 TTGATCGTGCCGTCCTCT For PCR screening CP3FMGG_06704 CATCGTGGACATCTTGGAG For PCR screening CP3RMGG_06704 CGAAACTTCTGGTGGTGAT For PCR screening CP4FMGG_00745 CTCCGTTGCGTCGTCTGT For PCR screening CP4RMGG_00745 TCTGGTCCGTCTTGCTGTT For PCR screening CP5FMGG_04068 ATCACAACCCTCCGAACCA For PCR screening CP5FMGG_04068 GCAAACCTGTCCTCGTAGTCC For PCR screening R-P1FMGG-00623 CGCATCCCAAGCCTGAAT For RT-PCR screening R-P1RMGG-00623 AGAACGGCGGGTGACAAG For RT-PCR screening R-P2FMGG_00871 AAGGGTCCGACGAGCAAA For RT-PCR screening R-P2RMGG_00871 CCTCCAACTCCACGGGTAT For RT-PCR screening R-P3FMGG_06704 GGAGTGGGAGGACAATGAA For RT-PCR screening R-P3RMGG_06704 GTCGCAATGGCAAGAACA For RT-PCR screening R-P4FMGG_00745 GGACCCAATACCCAACGAC For RT-PCR screening R-P4RMGG_00745 ACGGCTCATACGGCATAAA For RT-PCR screening
