Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae

Table 4

Conidiation, conidial germination, and appressorium formation of the wild Guy11 and mutants.

M. oryzae	Conidiation (×10^4 cm^−2)	Conidial germination (%)	Appressorium formation (%)
		4 h	12 h
Guy11
ΔMGG_00623
ΔMGG_00871
ΔMGG_06704
ΔMGG_00745

After incubation for 10 days on CM plates, conidia were collected using three 1 cm diameter discs of mycelium in water and counted with a haemacytometer under a microscope.
Conidial germination and appressorium formation were recorded after different time incubation of the conidial suspension on the hydrophobic surface of film as described previously. The letters “a” in each column are not significantly different, as estimated by Duncan’s test ().