Figure 2: Tyrosine phosphorylation of NR2B induces the development of chronic migraine and migraine attacks. The expression of NR2B-pTyr by western blots (a, b) and the levels of NO (c) by the nitric acid reductase method were increased in chronic migraine. And the increased NR2B-pTyr and NO levels were reversed by both low and high dosage of genistein (inhibitor of NR2B tyrosine phosphorylation) in CM rats. versus sham; versus CM-vehicle; for each group.