A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

Figure 2

Cross-reactivity assay and detection of clinical samples. Different combinations of pathogens DNA were used as templates (a–c); all of the specific amplification peaks were observed, presenting the gene-specific target amplicon without cross-amplification. The detection results of clinical samples (d–f). (a) The templates were nine target pathogens. (b) The templates were PRV, FDMV, PCV-2, PPV, RV, BVDV, Salmonella, Pm, MRSA, HPS, and S. suis. (c) The templates were PRRSV, JEV, CSFV, PEDV, RV, BVDV, Salmonella, Pm, MRSA, HPS, and S. suis. (d) The sample was PCV-2 positive. (e) The sample was PRV and PCV-2 positive. (f) The sample was PRRSV, JEV, and CSFV positive. (g) QX DNA size marker, 25–500 bp.

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