A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

<div>Sensitivity test of multiple PCR and PRV mono-PCR by QIAxcel; the detection limit of multiple PCR-QCES achieved approximately 104 copies/μL when all nine templates were present (a) and the detection limit of PRV mono-PCR achieved approximately 102 copies/μL (b). M: QX DNA size marker, 25–500 bp. Lanes A1–A6: the template mixtures were 102 copies/μL to 107 copies/μL, respectively; lanes B1–B5: 104 copies/μL to 100 copies/μL.</div>

BioMed Research International

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Figure 3

Figure 3: A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine 

Figure 3 | A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine 