A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

Table 2

Results of clinical samples detection.

Sample type/pathogens	Number of positive cases	Positive rate% (positive/sample)
The sample type
Visceral tissues	53	85.48% (53/62)
Abortus	11	78.57% (11/14)
Semen	4	17.39% (4/23)
Blood	9	20.00% (9/45)
Total	77	53.47% (77/144)
Pathogens
PRV	2	2.60%
PRRSV	8	10.39%
JEV	3	3.90%
FMDV	1	1.30%
CSFV	4	5.19%
PCV-2	12	15.58%
PEDV	5	6.49%
PPV	2	2.60%
PRV + PCV-2	5	6.49%
PRV + CSFV	2	2.60%
PCV-2 + PRRSV	17	22.08%
PCV-2 + CSFV	6	7.79%
PEDV + TGEV	3	3.90%
PRRSV + CSFV	2	2.60%
PCV-2 + PRRSV + CSFV	3	3.90%
CSFV + JEV + PRRSV	1	1.30%
FMDV + PRV + PCV-2 + PPV	1	1.30%

PRV: pseudorabies virus; CSFV: classic swine fever virus; JEV: Japanese encephalitis virus; PCV-2: porcine circovirus type 2; PRRSV: porcine reproductive and respiratory syndrome virus; PPV: porcine parvovirus. FMDV: foot-and-mouth disease virus. PEDV: porcine epizootic diarrhea virus. TGEV: transmissible gastroenteritis virus. At least one kind of virus was tested positive.