Evaluation of Antioxidant Potentials and α-Amylase Inhibition of Different Fractions of Labiatae Plants Extracts: As a Model of Antidiabetic Compounds Properties

Table 3

Percentage inhibition and kinetic parameters of α-amylase in plant extracts and their fractions.

Samples	Km mM	Vmax/min	α-Amylase inhibition%
α-Amylase without inhibitor	4.2 ± 0.004	0.12 ± 0.02
Acarbose	0.95 ± 0.02	0.04 ± 0.001	100
Ethanol extract of Zm	0.66 ± 0.01	0.05 ± 0.01	47.23 ± 1.2
Petroleum ether fraction of Zm	ND	ND	ND
Chloroform fraction of Zm	7.2 ± 0.02	0.01 ± 0.001	42.7 ± 0.69
Ethyl acetate fraction of Zm	1.4 ± 0.03	0.05 ± 0.001	55.5 ± 1.52
Butanol fraction of Zm	0.66 ± 0.01	0.05 ± 0.001	47.23 ± 1.2
Ethanol extract of Op	2.42 ± 0.01	0.09 ± 0.001	55.6 ± 0.25
Petroleum ether fraction of Op	4.85 ± 0.02	0.12 ± 0.005	53.3 ± 2.5
Chloroform fraction of Op	5.7 ± 0.0	0.12 ± 0.0005	58.5 ± 1.4
Ethyl acetate fraction of Op	2.4 ± 0.03	0.07 ± 0.001	99.4 ± 0.94
Butanol fraction of Op	2.23 ± 0.3	0.08 ± 0.005	65.3 ± 0.7
Ethanol extract of Sm	1.96 ± 0.01	0.07 ± 0.004	79.4 ± 1. 3
Petroleum ether fraction of Sm	4.6 ± 0.004	0.092 ± 0.01	42.08 ± 0.84
Chloroform fraction of Sm	3.6 ± 0.005	0.09 ± 0.03	24.2 ± 1.2
Ethyl acetate fraction of Sm	0.43 ± 0.03	0.01 ± 0.03	38.14 ± 1.04
Butanol fraction of Sm	1.2 ± 0.02	0.04 ± 0.02	45.6 ± 0.9

Zm: Zataria multiflora, Op: Otostegia Persica, Sm: Salvia mirzayanii, NA: nonactive.