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BioMed Research International
Volume 2017, Article ID 7348919, 9 pages
https://doi.org/10.1155/2017/7348919
Review Article

Bioengineered Plants Can Be a Useful Source of Omega-3 Fatty Acids

1State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
2Laboratory of Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China
3Department of Agriculture, Abdul Wali Khan University Mardan, Mardan, Pakistan

Correspondence should be addressed to Hu Chun-Mei; nc.ude.uajn@mchjjj

Received 30 October 2016; Accepted 17 January 2017; Published 21 February 2017

Academic Editor: Amit K. Rai

Copyright © 2017 Waleed Amjad Khan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. P. Insel, D. Ross, and K. McMahon, Discovering Nutrition, Jones & Bartlett Publishers, 2013.
  2. S. L. Pereira, A. E. Leonard, Y.-S. Huang, L.-T. Chuang, and P. Mukerji, “Identification of two novel microalgal enzymes involved in the conversion of the ω3-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid,” Biochemical Journal, vol. 384, no. 2, pp. 357–366, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. N. Ruiz-López, O. Sayanova, J. A. Napier, and R. P. Haslam, “Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants,” Journal of Experimental Botany, vol. 63, no. 7, pp. 2397–2410, 2012. View at Publisher · View at Google Scholar · View at Scopus
  4. J. Rombaldi Bernardi, R. de Souza Escobar, C. F. Ferreira, and P. Pelufo Silveira, “Fetal and neonatal levels of omega-3: effects on neurodevelopment, nutrition, and growth,” The Scientific World Journal, vol. 2012, Article ID 202473, 8 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. A. Leaf and P. C. Weber, “A new era for science in nutrition,” American Journal of Clinical Nutrition, vol. 45, no. 5, supplement, 1987, http://agris.fao.org/aos/records/US883244788. View at Google Scholar
  6. A. P. Simopoulos, “Human requirement for N-3 polyunsaturated fatty acids,” Poultry Science, vol. 79, no. 7, pp. 961–970, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. P. Moynihan, “Nutrition: the British nutrition foundation oral task force report—issues relevant to dental health professionals,” British Dental Journal, vol. 188, no. 6, pp. 308–312, 2000. View at Google Scholar · View at Scopus
  8. G. C. Burdge and P. C. Calder, “Conversion of α-linolenic acid to longer-chain polyunsaturated fatty acids in human adults,” Reproduction Nutrition Development, vol. 45, no. 5, pp. 581–597, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. WHO, Population Nutrient Intake Goals for Preventing Diet-Related Chronic Diseases, WHO, Geneva, Switzerland, 2003.
  10. I. Tetens, Scientific Opinion of the Panel on Dietetic Products, Nutrition and Allergies on a Request from the: Question No EFSA-Q-2008-269, European Food Safety Authority, 2008.
  11. I. Sioen, S. De Henauw, J. Van Camp, J.-L. Volatier, and J.-C. Leblanc, “Comparison of the nutritional-toxicological conflict related to seafood consumption in different regions worldwide,” Regulatory Toxicology and Pharmacology, vol. 55, no. 2, pp. 219–228, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. U. Gogus and C. Smith, “N-3 omega fatty acids: a review of current knowledge,” International Journal of Food Science and Technology, vol. 45, no. 3, pp. 417–436, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. B. J. Meyer, N. J. Mann, J. L. Lewis, G. C. Milligan, A. J. Sinclair, and P. R. C. Howe, “Dietary intakes and food sources of omega-6 and omega-3 polyunsaturated fatty acids,” Lipids, vol. 38, no. 4, pp. 391–398, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. P. C. Calder and P. Yaqoob, “Omega-3 polyunsaturated fatty acids and human health outcomes,” BioFactors, vol. 35, no. 3, pp. 266–272, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. A. P. DeFilippis, M. J. Blaha, and T. A. Jacobson, “Omega-3 fatty acids for cardiovascular disease prevention,” Current Treatment Options in Cardiovascular Medicine, vol. 12, no. 4, pp. 365–380, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. D. Kromhout, E. B. Bosschieter, and C. De Lezenne Coulander, “The inverse relation between fish consumption and 20-year mortality from coronary heart disease,” New England Journal of Medicine, vol. 312, no. 19, pp. 1205–1209, 1985. View at Publisher · View at Google Scholar · View at Scopus
  17. P. M. Kris-Etherton, F. B. Hu, E. Ros, and J. Sabaté, “The role of tree nuts and peanuts in the prevention of coronary heart disease: multiple potential mechanisms,” The Journal of Nutrition, vol. 138, no. 9, pp. 1746S–1751S, 2008. View at Google Scholar · View at Scopus
  18. C. Wang, W. S. Harris, M. Chung et al., “n-3 fatty acids from fish or fish-oil supplements, but not α-linolenic acid, benefit cardiovascular disease outcomes in primary- and secondary-prevention studies: a systematic review,” American Journal of Clinical Nutrition, vol. 84, no. 1, pp. 5–17, 2006. View at Google Scholar · View at Scopus
  19. V. M. Montori, A. Farmer, P. C. Wollan, and S. F. Dinneen, “Fish oil supplementation in type 2 diabetes: a quantitative systematic review,” Diabetes Care, vol. 23, no. 9, pp. 1407–1415, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. D. P. Rose, J. M. Connolly, and M. Coleman, “Effect of omega-3 fatty acids on the progression of metastases after the surgical excision of human breast cancer cell solid tumors growing in nude mice,” Clinical Cancer Research, vol. 2, no. 10, pp. 1751–1756, 1996. View at Google Scholar · View at Scopus
  21. D. P. Rose and J. M. Connolly, “Omega-3 fatty acids as cancer chemopreventive agents,” Pharmacology and Therapeutics, vol. 83, no. 3, pp. 217–244, 1999. View at Publisher · View at Google Scholar · View at Scopus
  22. D. P. Rose and J. M. Connolly, “Effects of dietary omega-3 fatty acids on human breast cancer growth and metastases in nude mice,” Journal of the National Cancer Institute, vol. 85, no. 21, pp. 1743–1747, 1993. View at Publisher · View at Google Scholar · View at Scopus
  23. A. J. Cockbain, G. J. Toogood, and M. A. Hull, “Omega-3 polyunsaturated fatty acids for the treatment and prevention of colorectal cancer,” Gut, vol. 61, no. 1, pp. 135–149, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. K. H. Weylandt, C.-Y. Chiu, B. Gomolka, S. F. Waechter, and B. Wiedenmann, “Omega-3 fatty acids and their lipid mediators: towards an understanding of resolvin and protectin formation. Omega-3 fatty acids and their resolvin/protectin mediators,” Prostaglandins and Other Lipid Mediators, vol. 97, no. 3-4, pp. 73–82, 2012. View at Publisher · View at Google Scholar · View at Scopus
  25. P. C. Calder, “n−3 Polyunsaturated fatty acids, inflammation, and inflammatory diseases,” The American Journal of Clinical Nutrition, vol. 83, no. 6, pp. S1505–1519S, 2006. View at Google Scholar · View at Scopus
  26. J. A. Conquer, M. C. Tierney, J. Zecevic, W. J. Bettger, and R. H. Fisher, “Fatty acid analysis of blood plasma of patients with Alzheimer's disease, other types of dementia, and cognitive impairment,” Lipids, vol. 35, no. 12, pp. 1305–1312, 2000. View at Publisher · View at Google Scholar · View at Scopus
  27. A. P. Simopoulos, “The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases,” Experimental Biology and Medicine, vol. 233, no. 6, pp. 674–688, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. C. J. Fabian, B. F. Kimler, B. K. Petroff et al., “High-dose omega-3 fatty acid supplementation to modulate breast tissue biomarkers in premenopausal women at high risk for development of breast cancer,” Cancer Research, vol. 31, p. 73, 2013. View at Google Scholar
  29. K. S. Bishop, S. Erdrich, N. Karunasinghe et al., “An investigation into the association between DNA damage and dietary fatty acid in men with prostate cancer,” Nutrients, vol. 7, no. 1, pp. 405–422, 2015. View at Publisher · View at Google Scholar · View at Scopus
  30. Z. Gu, J. Suburu, H. Chen, and Y. Q. Chen, “Mechanisms of omega-3 polyunsaturated fatty acids in prostate cancer prevention,” BioMed Research International, vol. 2013, Article ID 824563, 10 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. J. X. Kang and A. Liu, “The role of the tissue omega-6/omega-3 fatty acid ratio in regulating tumor angiogenesis,” Cancer and Metastasis Reviews, vol. 32, no. 1-2, pp. 201–210, 2013. View at Publisher · View at Google Scholar · View at Scopus
  32. E. M. Masko, E. H. Allott, and S. J. Freedland, “The relationship between nutrition and prostate cancer: is more always better?” European Urology, vol. 63, no. 5, pp. 810–820, 2013. View at Publisher · View at Google Scholar · View at Scopus
  33. A. P. Simopoulos, “Genetic variants in the metabolism of omega-6 and omega-3 fatty acids: their role in the determination of nutritional requirements and chronic disease risk,” Experimental Biology and Medicine, vol. 235, no. 7, pp. 785–795, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. G. P. Eckert, C. Franke, M. Nöldner et al., “Plant derived omega-3-fatty acids protect mitochondrial function in the brain,” Pharmacological Research, vol. 61, no. 3, pp. 234–241, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. S. F. Nabavi, S. Bilotto, G. L. Russo et al., “Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials,” Cancer and Metastasis Reviews, vol. 34, no. 3, pp. 359–380, 2015. View at Publisher · View at Google Scholar · View at Scopus
  36. T. Piccolo, “Framework analysis of fish waste to biodiesel production-Aquafinca. Case study,” 2008.
  37. D. R. Tocher, “Issues surrounding fish as a source of omega-3 long-chain polyunsaturated fatty acids,” Lipid Technology, vol. 21, no. 1, pp. 13–16, 2009. View at Publisher · View at Google Scholar · View at Scopus
  38. S. M. Hixson, “Fish nutrition and current issues in aquaculture: the balance in providing safe and nutritious seafood, in an environmentally sustainable manner,” Journal of Aquaculture Research & Development, vol. 5, no. 3, Article ID 1000234, 2014. View at Publisher · View at Google Scholar · View at Scopus
  39. R. Arthur, “Omega-3 sources,” Journal of Complementary Medicine, vol. 8, no. 3, pp. 28–48, 2009. View at Google Scholar · View at Scopus
  40. FAO, The State of World Fisheries and Aquaculture, Food and Agriculture Organization of the United Nations, Rome, Italy, 2010.
  41. U. R. Sumaila, A. Khan, R. Watson et al., “The World Trade Organization and global fisheries sustainability,” Fisheries Research, vol. 88, pp. 1–4, 2007. View at Google Scholar
  42. N. Ruiz-López, R. P. Haslam, M. Venegas-Calerón et al., “Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing,” Transgenic Research, vol. 21, no. 6, pp. 1233–1243, 2012. View at Publisher · View at Google Scholar · View at Scopus
  43. J. H. Lee, J. H. O'Keefe, C. J. Lavie, and W. S. Harris, “Omega-3 fatty acids: cardiovascular benefits, sources and sustainability,” Nature Reviews Cardiology, vol. 6, no. 12, pp. 753–758, 2009. View at Publisher · View at Google Scholar · View at Scopus
  44. J. R. Petrie, P. Shrestha, S. Belide et al., “Metabolic engineering Camelina sativa with fish oil-like levels of DHA,” PLoS ONE, vol. 9, no. 1, Article ID e85061, 2014. View at Publisher · View at Google Scholar · View at Scopus
  45. B. Qi, T. Fraser, S. Mugford et al., “Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants,” Nature Biotechnology, vol. 22, no. 6, pp. 739–745, 2004. View at Publisher · View at Google Scholar · View at Scopus
  46. G. Wu, M. Truksa, N. Datla et al., “Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants,” Nature Biotechnology, vol. 23, no. 8, pp. 1013–1017, 2005. View at Publisher · View at Google Scholar · View at Scopus
  47. M. Venegas-Calerón, O. Sayanova, and J. A. Napier, “An alternative to fish oils: metabolic engineering of oil-seed crops to produce omega-3 long chain polyunsaturated fatty acids,” Progress in Lipid Research, vol. 49, no. 2, pp. 108–119, 2010. View at Publisher · View at Google Scholar · View at Scopus
  48. E. Ryckebosch, C. Bruneel, R. Termote-Verhalle, K. Goiris, K. Muylaert, and I. Foubert, “Nutritional evaluation of microalgae oils rich in omega-3 long chain polyunsaturated fatty acids as an alternative for fish oil,” Food Chemistry, vol. 160, pp. 393–400, 2014. View at Publisher · View at Google Scholar · View at Scopus
  49. J. G. Bell, J. Pratoomyot, F. Strachan et al., “Growth, flesh adiposity and fatty acid composition of Atlantic salmon (Salmo salar) families with contrasting flesh adiposity: effects of replacement of dietary fish oil with vegetable oils,” Aquaculture, vol. 306, no. 1–4, pp. 225–232, 2010. View at Publisher · View at Google Scholar · View at Scopus
  50. J. A. Kralovec, S. Zhang, W. Zhang, and C. J. Barrow, “A review of the progress in enzymatic concentration and microencapsulation of omega-3 rich oil from fish and microbial sources,” Food Chemistry, vol. 131, no. 2, pp. 639–644, 2012. View at Publisher · View at Google Scholar · View at Scopus
  51. P. D. Nichols, B. Glencross, J. R. Petrie, and S. P. Singh, “Readily available sources of long-chain omega-3 oils: is farmed australian seafood a better source of the good oil than wild-caught seafood?” Nutrients, vol. 6, no. 3, pp. 1063–1079, 2014. View at Publisher · View at Google Scholar · View at Scopus
  52. D. Pauly, V. Christensen, S. Guénette et al., “Towards sustainability in world fisheries,” Nature, vol. 418, no. 6898, pp. 689–695, 2002. View at Publisher · View at Google Scholar · View at Scopus
  53. X. Qiu, “Biosynthesis of docosahexaenoic acid (DHA, 22:6-4, 7,10,13,16,19): two distinct pathways,” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 68, no. 2, pp. 181–186, 2003. View at Publisher · View at Google Scholar · View at Scopus
  54. P. W. Behrens and D. J. Kyle, “Microalgae as a source of fatty acids,” Journal of Food Lipids, vol. 3, no. 4, pp. 259–272, 1996. View at Publisher · View at Google Scholar · View at Scopus
  55. Y. Chisti, “Biodiesel from microalgae,” Biotechnology Advances, vol. 25, no. 3, pp. 294–306, 2007. View at Publisher · View at Google Scholar · View at Scopus
  56. K. Wright, C. Coverston, M. Tiedeman, and J. A. Abegglen, “Formula supplemented with docosahexaenoic acid (DHA) and arachidonic acid (ARA): a critical review of the research,” Journal for Specialists in Pediatric Nursing, vol. 11, no. 2, pp. 100–112, 2006. View at Publisher · View at Google Scholar · View at Scopus
  57. P. L. Sharpe, Q. Zhu, Z. Xue et al., “Production of omega-3 fatty acids via the fermentation of engineered strains of the oleaginous yeast Yarrowia lipolytica,” in Proceedings of the Annual Meeting and Exhibition, July 2009.
  58. W. R. Barclay, K. M. Meager, and J. R. Abril, “Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms,” Journal of Applied Phycology, vol. 6, no. 2, pp. 123–129, 1994. View at Publisher · View at Google Scholar · View at Scopus
  59. R. P. Haslam, N. Ruiz-Lopez, P. Eastmond, M. Moloney, O. Sayanova, and J. A. Napier, “The modification of plant oil composition via metabolic engineering—better nutrition by design,” Plant Biotechnology Journal, vol. 11, no. 2, pp. 157–168, 2013. View at Publisher · View at Google Scholar · View at Scopus
  60. S. S. Robert, S. P. Singh, X.-R. Zhou et al., “Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil,” Functional Plant Biology, vol. 32, no. 6, pp. 473–479, 2005. View at Publisher · View at Google Scholar · View at Scopus
  61. M. Kajikawa, K. Matsui, M. Ochiai et al., “Production of arachidonic and eicosapentaenoic acids in plants using bryophyte fatty acid Δ6-desaturase, Δ6-elongase, and Δ5-desaturase genes,” Bioscience, Biotechnology and Biochemistry, vol. 72, no. 2, pp. 435–444, 2008. View at Publisher · View at Google Scholar · View at Scopus
  62. S. Morais, R. B. Edvardsen, D. R. Tocher, and J. G. Bell, “Transcriptomic analyses of intestinal gene expression of juvenile Atlantic cod (Gadus morhua) fed diets with Camelina oil as replacement for fish oil,” Comparative Biochemistry and Physiology B: Biochemistry and Molecular Biology, vol. 161, no. 3, pp. 283–293, 2012. View at Publisher · View at Google Scholar · View at Scopus
  63. S. M. Hixson, C. C. Parrish, and D. M. Anderson, “Use of camelina oil to replace fish oil in diets for farmed salmonids and atlantic cod,” Aquaculture, vol. 431, pp. 44–52, 2014. View at Publisher · View at Google Scholar · View at Scopus
  64. T. C. Adarme-Vega, S. R. Thomas-Hall, D. K. Y. Lim, and P. M. Schenk, “Effects of long chain fatty acid synthesis and associated gene expression in microalga Tetraselmis sp.,” Marine Drugs, vol. 12, no. 6, pp. 3381–3398, 2014. View at Publisher · View at Google Scholar · View at Scopus
  65. M. D. Huynh and D. D. Kitts, “Evaluating nutritional quality of pacific fish species from fatty acid signatures,” Food Chemistry, vol. 114, no. 3, pp. 912–918, 2009. View at Publisher · View at Google Scholar · View at Scopus
  66. A. P. Kitson, A. C. Patterson, H. Izadi, and K. D. Stark, “Pan-frying salmon in an eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) enriched margarine prevents EPA and DHA loss,” Food Chemistry, vol. 114, no. 3, pp. 927–932, 2009. View at Publisher · View at Google Scholar · View at Scopus
  67. L.-T. Chuang, U. Bülbül, P.-C. Wen, R. H. Glew, and F. A. Ayaz, “Fatty acid composition of 12 fish species from the Black Sea,” Journal of Food Science, vol. 77, no. 5, pp. C512–C518, 2012. View at Publisher · View at Google Scholar · View at Scopus
  68. V. Loukas, C. Dimizas, V. J. Sinanoglou, and S. Miniadis-Meimaroglou, “EPA, DHA, cholesterol and phospholipid content in Pagrus pagrus (cultured and wild), Trachinus draco and Trigla lyra from Mediterranean Sea,” Chemistry and Physics of Lipids, vol. 163, no. 3, pp. 292–299, 2010. View at Publisher · View at Google Scholar · View at Scopus
  69. M. Heissenberger, J. Watzke, and M. J. Kainz, “Effect of nutrition on fatty acid profiles of riverine, lacustrine, and aquaculture-raised salmonids of pre-alpine habitats,” Hydrobiologia, vol. 650, no. 1, pp. 243–254, 2010. View at Publisher · View at Google Scholar · View at Scopus
  70. M. I. Gladyshev, E. V. Lepskaya, N. N. Sushchik et al., “Comparison of polyunsaturated fatty acids content in filets of anadromous and landlocked sockeye salmon oncorhynchus nerka,” Journal of Food Science, vol. 77, no. 12, pp. C1307–C1310, 2012. View at Publisher · View at Google Scholar · View at Scopus
  71. M. I. Gladyshev, N. N. Sushchik, G. A. Gubanenko, S. M. Demirchieva, and G. S. Kalachova, “Effect of boiling and frying on the content of essential polyunsaturated fatty acids in muscle tissue of four fish species,” Food Chemistry, vol. 101, no. 4, pp. 1694–1700, 2007. View at Publisher · View at Google Scholar · View at Scopus
  72. P. M. Kris-Etherton, D. S. Taylor, S. Yu-Poth et al., “Polyunsaturated fatty acids in the food chain in the United States,” American Journal of Clinical Nutrition, vol. 71, no. 1, pp. 179s–188s, 2000. View at Google Scholar
  73. I. Khozin-Goldberg, S. Didi-Cohen, I. Shayakhmetova, and Z. Cohen, “Biosynthesis of eicosapentaenoic acid (EPA) in the freshwater eustigmatophyte Monodus subterraneus (Eustigmatophyceae),” Journal of Phycology, vol. 38, no. 4, pp. 745–756, 2002. View at Publisher · View at Google Scholar · View at Scopus
  74. H. Hu and K. Gaol, “Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources,” Biotechnology Letters, vol. 25, no. 5, pp. 421–425, 2003. View at Publisher · View at Google Scholar · View at Scopus
  75. D. Pal, I. Khozin-Goldberg, Z. Cohen, and S. Boussiba, “The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsissp.,” Applied Microbiology and Biotechnology, vol. 90, no. 4, pp. 1429–1441, 2011. View at Publisher · View at Google Scholar · View at Scopus
  76. V. Patil, T. Källqvist, E. Olsen, G. Vogt, and H. R. Gislerød, “Fatty acid composition of 12 microalgae for possible use in aquaculture feed,” Aquaculture International, vol. 15, no. 1, pp. 1–9, 2007. View at Publisher · View at Google Scholar · View at Scopus
  77. J. Van Wagenen, T. W. Miller, S. Hobbs, P. Hook, B. Crowe, and M. Huesemann, “Effects of light and temperature on fatty acid production in Nannochloropsis salina,” Energies, vol. 5, no. 3, pp. 731–740, 2012. View at Publisher · View at Google Scholar · View at Scopus
  78. M. Sang, M. Wang, J. Liu, C. Zhang, and A. Li, “Effects of temperature, salinity, light intensity, and pH on the eicosapentaenoic acid production of Pinguiococcus pyrenoidosus,” Journal of Ocean University of China, vol. 11, no. 2, pp. 181–186, 2012. View at Publisher · View at Google Scholar · View at Scopus
  79. R. A. Bhosale, M. P. Rajabhoj, and B. B. Chaugule, “Dunaliella salina Teod. as a prominent source of eicosapentaenoic acid,” International Journal on Algae, vol. 12, no. 2, pp. 185–189, 2010. View at Publisher · View at Google Scholar · View at Scopus
  80. A. Mendes, T. L. Da Silva, and A. Reis, “DHA concentration and purification from the marine heterotrophic microalga Crypthecodinium cohnii CCMP 316 by winterization and urea complexation,” Food Technology and Biotechnology, vol. 45, no. 1, pp. 38–44, 2007. View at Google Scholar · View at Scopus
  81. I. Lang, L. Hodac, T. Friedl, and I. Feussner, “Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection,” BMC Plant Biology, vol. 11, article 124, 2011. View at Publisher · View at Google Scholar · View at Scopus
  82. M. L. Hamilton, S. Powers, J. A. Napier, and O. Sayanova, “Heterotrophic production of omega-3 long-chain polyunsaturated fatty acids by trophically converted marine diatom phaeodactylum tricornutum,” Marine Drugs, vol. 14, no. 3, article no. 53, 2016. View at Publisher · View at Google Scholar · View at Scopus
  83. F. Guihéneuf, V. Mimouni, L. Ulmann, and G. Tremblin, “Combined effects of irradiance level and carbon source on fatty acid and lipid class composition in the microalga Pavlova lutheri commonly used in mariculture,” Journal of Experimental Marine Biology and Ecology, vol. 369, no. 2, pp. 136–143, 2009. View at Publisher · View at Google Scholar · View at Scopus
  84. J. R. Petrie, P. Shrestha, M. P. Mansour, P. D. Nichols, Q. Liu, and S. P. Singh, “Metabolic engineering of omega-3 long-chain polyunsaturated fatty acids in plants using an acyl-CoA Δ6-desaturase with ω3-preference from the marine microalga Micromonas pusilla,” Metabolic Engineering, vol. 12, no. 3, pp. 233–240, 2010. View at Publisher · View at Google Scholar · View at Scopus
  85. J. R. Petrie and S. P. Singh, “Expanding the docosahexaenoic acid food web for sustainable production: engineering lower plant pathways into higher plants,” AoB PLANTS, vol. 2011, Article ID plr011, 2011. View at Publisher · View at Google Scholar · View at Scopus
  86. A. J. Kinney, E. B. Cahoon, H. G. Damude, W. D. Hitz, C. W. Kolar, and Z. Liu, “Production of very long chain polyunsaturated fatty acids in oilseed plants,” Patent WO., vol. 71467:A2., 2004.
  87. B. Cheng, G. Wu, P. Vrinten, K. Falk, J. Bauer, and X. Qiu, “Towards the production of high levels of eicosapentaenoic acid in transgenic plants: the effects of different host species, genes and promoters,” Transgenic Research, vol. 19, no. 2, pp. 221–229, 2010. View at Publisher · View at Google Scholar · View at Scopus