Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture
Morphology of cultured hAMSCs and hACLFs; the proliferative curve of cells in each group, as determined by CCK-8. Primary cultures of hAMSCs and hACLFs were cultured to the third passage (a) (original magnification 40x). Proliferation of hAMSCs in each group, as determined by the CCK-8 method showed that cells in Group E multiplied faster than those in Group C and other groups and that cells in Group B grew most slowly (b). The proliferative curves of hAMSCs and hACLFs based on the CCK-8 method showed a doubling time for hAMSCs and hACLFs as high as 32.5 h and 49.8 h, respectively; the proliferation rate of hAMSCs was significantly higher than that of hACLFs (c) (, scale bar in (a) = 200 m).
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