Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture

Table 2

Primer sequences for genes associated with hACLFs.

Target	Primer sequence ()	Product length (bp)
COL1-A1	Forward: TCCGACCTCTCTCCTCTGAA; Reverse: TGCTTTGTGCTTTGGGAAGT	121
COL3-A1	Forward: TCAGGGTGTCAAGGGTGAA; Reverse: CAGGGTTTCCATCTCTTCCA	131
Fibronectin	Forward: ATCACCCTCACCAACCTCAC; Reverse: TCCCTCGGAACATCAGAAAC	122
Tenascin-C	Forward: GGCAGGTGTCTTTCTTGCTT; Reverse: ACTGGCTGGTTCTCTTCTGG	120
MMP-2	Forward: TATGGCTTCTGCCCTGAGAC; Reverse: CACACCACATCTTTCCGTCA	142
LOX-1	Forward: TTACCCAGCCGACCAAGATA; Reverse: CCTTCAGCCACTCTCCTCTG	122
GAPDH	Forward: CATGTTTGTGATGGGCGTGAA; Reverse: AATGCCGAAGTGGTCGTGGAT	70