In Vitro Plant Regeneration from Commercial Cultivars of Soybean

Figure 1

Tissue culture steps in development of soybean plants (cv. Bunya as a representative); (a) soybean seeds on germination medium; (b) 6-day-old seedlings; (c, d, e) half split hypocotyl, complete hypocotyl, and cotyledonary node explants on shoot induction medium (day 0); (f, g, h) half split hypocotyls, complete hypocotyls, and cotyledonary explants on shoot induction medium after 15 days; (i, j, k) regenerated shoots from half split hypocotyl, complete hypocotyl, and cotyledonary node explants after 45 days; (l, m, n) shoots from half split hypocotyl, complete hypocotyl, and cotyledonary node explants on rooting medium.