GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells

Figure 5

The GosB-dependent protection from rosiglitazone- (ROSI-) induced cell apoptosis in mouse mammary gland epithelial cells (MEC). The MEC were transfected with Ad-GosB or Ad-siGosB for 48 hours and subsequently exposed to 10 μM rosiglitazone (ROSI) for 24 hours. Cell apoptosis was determined by Hoechst 33342/PI dual staining assays (a) and Annexin V-FITC/PI binding followed by flow cytometry (b). Values are means ± SEM for three individuals. Different lowercase letters (a, b) indicate significant (P < 0.05) differences.

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(b)

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