GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells

Figure 7

The GosB prevents mitochondrial calcium overload and subsequent apoptotic events. (a) Confocal laser scanning microscopic examination of MEC loaded with Rhod-2 AM showing mitochondrial Ca²⁺. (b–d) The MEC were transfected with Ad-GosB for 48 hours and subsequently exposed to 4 mM CaCl₂ for 24 hours. Cell apoptosis was determined by Annexin V-FITC/PI binding followed by flow cytometry. The mRNA expression was analyzed by qRT-PCR. Different lowercase (a–c) and uppercase letters (A–C) indicate significant (P < 0.05) differences.

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