Estrogen Replacement Regulates Vaginal Innervations in Ovariectomized Adult Virgin Rats: A Histological Study

Figure 1

Immunohistochemical photomicrographs of sections taken from vaginal tissues. Sections were obtained from rats in SHAM group (a), OVX group (b), E1 (0.4 mg/kg/d (c)), E2 (0.8 mg/kg/d (d)), E3 (1.6 mg/kg/d (e)), and E4 (3.2 mg/kg/d (f)). Pan-neuronal marker PGP9.5 immunostaining: small nerve fibers are indicated by small blue arrows. The scale bar in (a)–(f) represents 50 μm (200x magnification).

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