Estrogen Replacement Regulates Vaginal Innervations in Ovariectomized Adult Virgin Rats: A Histological Study

Figure 2

The effects of estrogen on vaginal innervation in rats. (a) Pan-neuronal marker PGP9.5 immunostaining: vaginal tissue sections from lamina propria layer of SHAM group rats. Nerve bundles are indicated by white arrows, while small nerve fibers are indicated by small blue arrows. Scale bar represents 30 μm (400x magnification). (b) Quantitative analysis of nerve density in the rats’ vagina: the Sham-operated (SHAM), the ovariectomy (OVX), E1 (0.4 mg/kg/d), E2 (0.8 mg/kg/d), E3 (1.6 mg/kg/d), and E4 (3.2 mg/kg/d) groups. for each group. for ; for ; for versus SHAM group (control group) and for versus OVX group (model).

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