Quantitative PCR and Digital PCR for Detection of Ascaris lumbricoides Eggs in Reclaimed Water

Table 2

Detection of A. lumbricoides by dPCR from reclaimed water seeded with different amounts of A. lumbriocoides eggs.

Protocol  1 (for 500 mL)			Protocol  2 (for 10 L)
Number of eggs	Mean; ITS-1 copies μL^−1	Result	Number of eggs	Mean; ITS-1 copies μL^−1	Result
1	4.35	Presence	10	—	NA
1	—	Absence	10	—	NA
1	—	Absence	10	—	NA
1	1.12	Presence	10	—	NA
1	7.14	Presence	10	—	NA
Average ± SD
5	13.25	Presence	20	—	NA
5	27.22	Presence	20	—	NA
5	23.77	Presence	20	—	NA
5	41.37	Presence	20	—	NA
5	7.05	Presence	20	—	NA
Average ± SD
10	16.36	Presence	50	—	NA
10	50.00	Presence	50	—	NA
10	23.30	Presence	50	—	NA
10	39.37	Presence	50	—	NA
10	28.74	Presence	50	—	NA
Average ± SD

Presence: more than one copy μL⁻¹. Absence: not detected; NA: no amplification.