Responders to Platelet-Rich Plasma in Osteoarthritis: A Technical Analysis

Table 3

Technical characteristic of the PRP preparation, quality, and injection assessed and reported by each author. Ref = references; inj = number of injections; inter = time interval between injections (weeks); vol = volume of PRP injected (mL); tech = preparation technique (name or description of the technique); speed = centrifugation speed (in g or RPM); num = number of centrifugations; time = time after blood puncture before injection; anticoag = anticoagulation; concentr = platelet concentration; WBC = leukocytes; activ = platelet activation; tampon = NaHCO₃; rehab = rehabilitation protocol; Mish = Mishra classification; PAW = PAW classification; storage: storage method. NM = not mentioned.

(a)

Ref	Inj	Inter	Vol	Tech	Speed	Num	Time	Anticoag	Concentr
Vaquerizo et al. [25]	3	2	8	Endoret	If like Sanchez: 580 g/8 min (=1861 rpm if  cm)	1	8 min centrifugation + 20 min preparation	3,8% citrate	<5x baseline (via Laudy), value NM
Patel et al. [26]	1 or 2	3	8	Centrifugation: BIOAIR Safe flow 1.2, Euroclone, Siziano, Italy	1500 rpm, 15 min	1	1 to 2 h (30 min preparation)	CPDA 1 = citrate, phosphate, dextrose, adenine	310.14 × 10^3/mL
Cerza et al. [28]	4	1	5.5	ACP	NM, Arthrex brochure: 1500 rpm, 5 min	1	NM	1 ml sodium citrate	>5x baseline (via Laudy), value NM (2 to 3 times baseline, brochure Arthrex)
Say et al. [30]	1	—	2.5	Endoret	1800 rpm, 8 min	1	30 min	3,2% sodium citrate	4x baseline, 818 520 mean value
Acosta-Olivo et al. [20]	2	2	5	GPS III Platelet Concentrate Separation Kit with ACD-A (Biomet, USA)	NM	NM	NM	NM	NM
Wang-Saegusa et al. [37]	3	2	5	Endroret	1800 rpm, 8 min	1	NM	Sodium citrate	NM
Halpern et al. [40]	1	—	6	MTF Cascade system	NM	NM	NM	NM	NM
Filardo et al. [27]	3	1	5	Double centrifugation	1480 rpm, 6 min and then 2 3400, 15 min	2	NM	NM	5x baseline (via Laudy), value NM
Sampson et al. [36]	3	4	6	GPS III Platelet Concentration System (Biomet Biologics, Warsaw, IN)	15 mins at 1700 g (Model 755 VES, The Drucker Company, Philipsburg, PA) = 3185 rpm if  cm	NM	NM	6 ml citrate dextrose	NM
Napolitano et al. [39]	3	1	5	Specific Fibrin Polymer 2 test-tube from RegenLab®, simple centrifugation	3100 rpm, 8 min	1	NM	NM	Platelet concentration factor: 2.3 (minimum 0.17, maximum 5.23)
Filardo et al. [21]	3	1	5	Double centrifugation	1480, 6 min and then 3400, 15 min	2	NM	NM	4.6 ± 1.4 times

(b)

Ref	RBC	WBC	Activ	Tampon	Rehab	Mish	PAW	Storage
Vaquerizo et al. [25]	NM	No	yes: CaCl2 400 µl	NM	NM	4B	P2Bβ (Delong et al.)	Blood extracted each time
Patel et al. [26]	No	No	1 ml CaCl2 for 4 ml prp	NM	Mobilisation 5–10 min then SLR, static quads, hamstring reinforcement	4B	P2Bβ	Blood extracted each time
Cerza et al. [28]	NM	None	No	NM	No	3A	P2Bβ (Delong et al.)	Blood extracted each time
Say et al. [30]	No	None or a few	yes: 50 µl CaCl2 for 1 ml prp = 5,5%	NM	Rest, paracetamol and cryotherapy, no NSAIDs	4B	P2Bβ (Delong et al.)	—
Acosta-Olivo et al. [20]	NM	NM	NM	NM	Relative rest 24 h, physical therapy: isometric and isotonic contractions	3A (Kaux et al.)	P2-4Aα (Delong et al.)	Blood extracted each time
Wang-Saegusa et al. [37]	NM	No	10% CaCl2	NM	NM	4B (Endoret)	P2Bβ (Delong et al.)	Not mentioned, freeze?
Halpern et al. [40]	NM	NM	NM	NM	NM	3 or 4B (activation?) (Delong et al.)	P2Bβ (Delong et al.)	—
Filardo et al. [27]	NM	Yes: 1,2x baseline	NM	NM	Rest, light activity	2A	P3Ax	Freeze −30°
Sampson et al. [36]	NM	NM	6 ml prp + 0.6 ml of a 1000 U/ml bovine thrombin suspension in 10% calcium chloride solution	NM	Passive flexion and extension, 3 times, and then 10 mins of rest, acetaminophen and hydrocodone against pain, relative rest 24 H after injection, no standardised protocol	3A (Kaux et al.)	P2-4Aα (Delong et al.)	Blood extracted each time
Napolitano et al. [39]	No	NM	Calcium gluconate 10%	NM	No NSAIDs, no heavy physical activity for lower limbs for 2 weeks	2B (Magalon et al.)	P2Ax (Magalon et al.)	Blood extracted each time
Filardo et al. [21]	NM	Yes: 1,1 ± 0,5	10% CaCl2	NM	NM	2B	P3Ax	Freeze −30°