MET18 Deficiency Increases the Sensitivity of Yeast to Oxidative Stress and Shortens Replicative Lifespan by Inhibiting Catalase Activity

Figure 3

Effects of CTT1 or CTA1 on oxidative stress resistance in MET18/met18Δ cells. Catalase activity (a) and the intracellular H₂O₂ levels (b) under unstressed conditions were determined and compared among pAUR123-transfected WT strain (WT + p), pAUR123-, and CTT1- and CTA1-transfected MET18/met18Δ mutants ((Δ + p), (Δ + CTT1), and (Δ + CTA1), resp.). versus WT cells (). (c) Tenfold dilution series of WT + p, Δ + p, Δ + CTT1, or Δ + CTA1 were spotted on YPD plates with or without the stressors, as indicated, and incubated at 30°C for 2~3 days to detect the cellular response. Results shown are representative of three independent experiments.

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