Research Article
MET18 Deficiency Increases the Sensitivity of Yeast to Oxidative Stress and Shortens Replicative Lifespan by Inhibiting Catalase Activity
Table 1
Yeast strains in this study.
| Strain name | Genotype | Comments | Source |
| BY4743 | MATa/MATαhis3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2/lys2Δ0 met15Δ0/met15 ura3Δ0/ura3Δ0 | Wild-type strain | Gift from Matt Kaeberlein | GDMUB1001 | BY4743 MET18::URA3 | Deletion of one copy of MET18 in BY4743 | This experiment | GDMUB1002 | BY4743 + pAUR123 | BY4743 harboring pAUR123 | This experiment | GDMUB1003 | BY4743MET18::URA3 + pAUR123 | GDMUB1001 harboring pAUR123 | This experiment | GDMUB1004 | BY4743MET18::URA3 + pAUR123-CTA1 | GDMUB1001 harboring pAUR123-CTA1 | This experiment | GDMUB1005 | BY4743MET18::URA3 + pAUR123-CTT1 | GDMUB1001 harboring pAUR123-CTT1 | This experiment |
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