Expression and Immunogenicity of Two Recombinant Fusion Proteins Comprising Foot-and-Mouth Disease Virus Structural Protein VP1 and DC-SIGN-Binding Glycoproteins

Figure 1

Design of the recombinant transfer vector for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins and confirmation of the recombinant transfer vector and baculovirus bacmids. indicates the DNA size marker. (a) Sites of insertion of the coding sequences for the VP1 protein and the VP1-gp120 and VP1-E2 fusion proteins in the baculovirus transfer vector pFastBac 1. (b) The recombinant transfer vectors pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 were digested with the restriction endonucleases BamHI and HindIII and subjected to agarose gel electrophoresis. Lanes 1, 3, and 5: undigested plasmids. Lanes 2, 4, and 6: pFastBac-VP1, pFastBac-VP1-gp120, and pFastBac-VP1-E2 digested with BamHI and HindIII, respectively. (c) The recombinant bacmids rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2 were amplified by PCR with the M13 universal primers, and the PCR products were subjected to agarose gel electrophoresis. Lanes 1, 2, and 3: PCR products from rBacmid-VP1, rBacmid-VP1-gp120, and rBacmid-VP1-E2, respectively.

(a)

(b)

(c)