Review Article

Circulating MicroRNAs as Potential Biomarkers of Atrial Fibrillation

Table 2

A review of sample preparation protocols and methods of miRNA detection in circulating miRNA.

AuthorsSample typeSample processing conditionsMethod of RNA isolationMethod of miRNA detection

Hoffmann et al., 2016Plasma<4 h of collection; centrifugation 4000 rpm, 20 min RTmiRNeasy kit (Qiagen)qRT-PCR
Nishi et al., 2013PlasmaNot describedmirVana™ PARIS RNA isolation kit (Ambion)qRT-PCR
McManus et al., 2014Whole bloodNot describedPAXgene Blood RNA Kit (PAXgene)qRT-PCR, BioMark dynamic array
McManus et al., 2015PlasmaCentrifugation 8,000 ×g, 5 minmiRCURY RNA Isolation Kit (Exiqon)qRT-PCR
Dawson et al., 2013Plasma<4 h of collection; centrifugation 4000 rpm, 20 min RTmirVana PARIS RNA isolation kit (Ambion)qRT-PCR
Goren et al., 2014Platelets and serumPlatelets: stand for 2 to 9.5 h of collection; centrifugation 1,800 ×g, 30 min RT; serum: stand for 2 to 9.5 hmirVana PARIS RNA isolation kit (Ambion)qRT-PCR
Wei et al., 2015SerumCentrifugation 3,000 ×g, 10 min RTmiRNeasy kit (Qiagen)qRT-PCR
Harling et al., 2017SerumCentrifugation 5,000 ×g, 6 min at RTNot describedqRT-PCR
Liu et al., 2012Plasma<20 min of collection; two-step centrifugation (not described)mirVana PARIS RNA isolation kit (Ambion)MPSS and qRT-PCR
Liu et al., 2016Plasma<1 h of collection; centrifugation 2,000 ×g, 10 min 4°CmiRNA extraction kit (BioTeke)Solexa sequencing and qRT-PCR
Lu et al., 2015PlasmaCentrifugation 3000 rpm, 10 minmirVana PARIS RNA isolation kit (Ambion)miRCURY™ LNA chip
Soeki et al., 2016PlasmaNot describedTRIzol LS RNA Isolation Kit (Invitrogen)qRT-PCR

RT, room temperature; qRT-PCR, quantitative real-time polymerase chain reaction; MPSS, massively parallel signature sequencing.