Innervation of the Human Cavum Conchae and Auditory Canal: Anatomical Basis for Transcutaneous Auricular Nerve Stimulation

Figure 6

Double immunofluorescence for S100 protein (green fluorescence) and myelin-basic protein (red fluorescence) in nerves of MAEC placed out of the cartilage ((a)–(e)) or inside the auricular cartilage of the cavum conchae ((f) and (g)). Colocalization of S100 protein and myelin-basic protein results in yellow fluorescence (c); (d) and (e) are details of the square in (c). Objective 40x/1.25 oil; pinhole airy 1, XY resolution 156 nm and Z resolution 334 nm. Scale bar common for (a)–(c) and (f)-(g); scale bar common for (d) and (e).

(a)

(b)

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(e)

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(g)