Research Article
The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional Profile In Vivo
Figure 2
Expression and cellular localisation of keratin 6b mRNA. (a) In situ RT-PCR showing the cellular localisation of mRNA in nonwounded, and 72 h wounded dorsal skin evaluated in 1.5-month-old NTG, K14E6, and K14E6Δ146-151 transgenic mice. Magnification: 20x. Dashed line: basal membrane. Ep: epidermis, Sb: suprabasal stratum; and b: basal stratum. (b) End-point RT-PCR showing the amplification products of K6b and GAPDH genes. (c) The RT-qPCR assay is showing the relative expression of Krt6 using the amount of target = equation; Krt6 RT-qPCR was performed as biological replicates. Positive control for (b) and (c) refers to 72 h wounded skin samples. Magnification: 20x.
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