The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional Profile In Vivo

Figure 4

CD34/Krt15 epidermal stemness markers and reepithelization/wound closure assays. (a) Immunofluorescence assay of nonwounded skin showing the expression and colocalization of CD34 (FITC-signal) and K5 (TRITC-signal) stemness markers. (b) Western blot of CD34 evaluated in total protein extracts of nonwounded skin samples. The graph shows the result of independent biological replicates. (c) Epithelization assay performed in 72, 96, and 120 h wounded dorsal skin indicating the percentage of complete epithelization for each mice strain. (d) Wound closure measure 28 days after wounding evaluated on ears. The hole diameter was measured with a micro ruler using a stereoscopic microscope. Magnification of (a): 40x.

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