Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Genes</td><td class="align_center">Primer sequences</td><td class="align_center">Annealing temperature (°C)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left"><i>β</i>-Actin</td><td class="align_center">Forward: CGTTGACATCCGTAAAGACCTC<br/>Reverse: TAGGAGCCAGGGCAGTAATCT</td><td class="align_center">58</td></tr><tr><td class="align_left">CD31</td><td class="align_center">Forward: GATCTCCATCCTGTCGGGTAAC<br/>Reverse: GTGTCATTCACGGTTTCTTCGT</td><td class="align_center">58</td></tr><tr><td class="align_left"><i>α</i>-SMA</td><td class="align_center">Forward: CAACCCCTATACAACCATCACAC<br/>Reverse: CCCAAACTGCTTGCGTAACC</td><td class="align_center">58</td></tr><tr><td class="align_left">VEGF</td><td class="align_center">Forward: ATCTTCAAGCCGTCCTGTGTG<br/>Reverse: AGGTTTGATCCGCATGATCTG</td><td class="align_center">58</td></tr><tr><td class="align_left">NG2</td><td class="align_center">Forward: TTACCTTGGCCTTGTTGGTC<br/>Reverse: GATGATCTGTTTGGCCTGCT</td><td class="align_center">58</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

The primer sequences for each primer used in the real-time RT-PCR. The primer sequences and annealing temperature of CD31, <i>α</i>-SMA, VEGF, NG2, and <i>β</i>-actin.

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Table 2

Table 2: Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect 

Table 2 | Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect 