Hibiscus syriacus Extract from an Established Cell Culture Stimulates Skin Wound Healing

Figure 1

Effect of Hibiscus syriacus ethanolic extract (HSEE) on wound healing. (a) Confluent HaCaT cells were wounded and then treated with HSEE at the indicated concentrations into the 0.5% FBS-containing medium. TGF β at concentration of 2.5 ng/ml was used as positive control. At times 0 and 24 h, phase-contrast pictures of the wounds at four different locations were taken. (b) The relative migration of the cells was expressed as % of wound repair compared to control. Each column value represents the average of three experiments and error bars indicate standard deviations. value < 0.05 is represented by . (c) Confluent HDF cells were wounded and then treated with HSEE at indicated concentrations for 7 h in 0.5% FBS-containing medium. TGFβ at concentration of 2.5 ng/ml was used as positive control. Mitomycin C (10 μg/ml) was included in the media to prevent cell proliferation. At times 0 and 7 h, phase-contrast pictures of the wounds at four different locations were taken. (d) For each condition, the unclosed cell-free areas at time 0 were compared to those at 7 h by using the software Image J. The relative migration of the cells was expressed as % of wound repair compared to control. Each column value represents the average of three experiments and error bars indicate standard deviations. P value < 0.05 is represented by . value < 0.001 is represented by .

(a)

(b)

(c)

(d)