Experimental Verification of CAPN1 and CAST Gene Polymorphisms in Different Generations of Da-Heng Broilers

Table 1

Primers for screening in the chicken CAPN1 and CAST genes.

	Primers	Sequence of the primers	Amplified length	Binding regions	Tm (°C)
CAPN1	P1	F: TCA CCT CAC GTG CCT CTC TCA	217	Exon 5	58.0
		R: CGG AAC ACT TAC GTC GAT
	P2	F: AGG GGT AGG GTA ATA GAA CTA	233	Exon 6	58.0
		R: ACC GCC AGC CAT CAA AT
	P3	F: CCT CCT TCC TCC TCA GAC AAA	191	Exon 16	55.0
		R: CAGCCT TGG CAC AAC TAG AGA
	P4	F: TCAGGACACTGG TGT TCA ATA	212	Intron 3	55.0
		R: GGA AAG GGT GTA GTG GTA C
CAST	P5	F: AAT ACA GGG TCA CAT CG	239	Exon 8	56.0
		R: AAA GAA ACA TTC CCT GA
	P6	F: AAA CGA GAA GGT AGC C	291	Exon 11	55.0
		R: CTG GTA TCT TTG GAA GAC ATA
	P7	F: CCA AAA GTA GAT GAA CAT TCT	249	Intron 11	48.0
		R: GCT TCT ATT AAT TCC TAC CT