Experimental Verification of CAPN1 and CAST Gene Polymorphisms in Different Generations of Da-Heng Broilers

Table 3

The Hardy-Weinberg equilibriumof CAPN1 and CAST gene mutation in the ninth generation.

Markers	Position	ObsHET	ExptHET	Allele change	Amino acids change	HWE ()	MAF
SNP1	3535	0.439	0.5	G>A	215 Leu > Leu	0.150	0.488
SNP2	7198	0.396	0.44	C>A	427 Glu > His	0.262	0.326
SNP3	7324	0.488	0.473	G>A	489 Val > Val	0.850	0.384
SNP4	9950	0.53	0.496	G>A	932 Cys > Arg	0.357	0.639
SNP6	37868	0.488	0.498	G>A	335 Arg > His	0.882	0.470

Notes. ObsHET, observed heterozygosity; ExptHET, expected heterozygosity; HWE, Hardy-Weinberg equilibrium; MAF, minimum allele frequency.