Figure 4: RT and real-time PCR analysis of candidate genes related to brain metastasis identified from the human transcriptome analysis. The abundance of CXCR4, TNFSF4, PLLP, VCAM1, SLC8A2, and SLC7A11 mRNAs in the brain of individual mice (#1, #2) with metastases formed by 231-Luc, MeWo, or WM3734 cells, or in primary tumors formed by 231-Luc cells (mammary fat pad) or by MeWo cells (subcutaneous) in individual mice (#1, #2) were normalized by GAPDH mRNA. Data are means ± SD of triplicate determinations. , , , and . NS: not significant.